Projects Under Way

Genome Engineering

GENETICS 

Prevention is always better than cure, simply because some of the diseases cannot be cured. They can only be prevented.

Genetic disorders are hard to prevent because the process of identification of Mutant Genotypes which are responsible for the disorder are very complex, time consuming and very expensive.

Nowadays more and more diseases are known to have some kind of genetic linkage. Certain type of cancers, familial type of diseases and many other diseases are linked to our genome itself. As our knowledge about diseases are increasing day by day, diagnosis & treatments are also improving exponentially.


Diagnosis : There are various methods to diagnose a disease. Genetic sequencing is one of the most advanced & accurate method used for determining genetic variations including mutations. But the problem with  sequencing is that it is a  very expensive process. And like any other methods of diagnosis, it is also not 100% accurate. There can be a minimal chances of errors or false positives or false negatives. 
As the technology is going through rapid advancement & breakthrough discoveries, we now have some very accurate & less expensive methods like Whole Exome Sequencing used in products like B-EDWIG & others. With products like B-EDWIG, an individual can  now identify any  possible genetic variations in his/her DNA which can lead to a disease in the future for that individual or for his/her generation to come. 

Treatment : As our diagnostic methods improves exponentially, the treatment methods are also undergoing some revolutionary advancements. The best treatment methods in development or already available now for genetic disorders includes gene therapy, DNA editing and targeted therapy. 


CRISPR method; CRISPR is an abbreviation of Clustered Regularly Interspaced Short Palindromic Repeats.  It has a huge potential in treating genetic disorders because of its precise cutting process. 


What we are trying to develop;
At B-Aegis we are working hard on developing a precise & potent DNA editing technology which is based on the Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 , the B-CRISPR/Cpf1 method. 
Cpf1 is a novel class of CRISPR-Cas DNA endonucleases,
with a wide range of activity across different
eukaryotic systems.

Why we are considering Cpf1 over Cas9?

Cas9 requires two RNA molecules to cut DNA while Cpf1 needs one. The proteins also cut DNA at different places, offering us more options when selecting an editing site. Cas9 cuts both strands in a DNA molecule at the same position, leaving behind ‘blunt’ ends. Cpf1 leaves one strand longer than the other, creating 'sticky' ends that are easier to work with. Cpf1 appears to be more able to insert new sequences at the cut site, compared to Cas9. 

We have demonstrated multiplex gene editing by Mb3Cpf1 using a single trans-activating  crRNA array in the lab, we have edited four genes in mammalian cells & we are focusing on developing the method in much wider scale. 

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Thiruvananthapuram, Kerala, India

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